u937 cell line

u937 cell line

Undifferentiated U937 cells produced no detectable IRAP protein. Ethical Standards for Obtaining Human Materials, Pleura/pleural effusion, lymphocyte, Myeloid, Nucleic Acids, Proteins and Cell Extracts, The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC. The procedure yields in approximately 700 µl of lysate (∼5 mg protein) recovered from 108 U937 cells. Quality Accreditations. Contact Us Cells are kept for 10 min at 4 °C, the temperature of all subsequent steps, and are then homogenized by 40 passages through a 26-gauge needle. LFM-A13, 13, was first described as a Btk inhibitor more than 10 years ago [67], although its poor Btk activity (IC50 = 2.5–17 μM) and selectivity [68] require that caution be taken in interpreting functional effects. The use of U937 cells in studies of cell-cell attachment in vitro are facilitated by the fact that this monocytic cell line does not adhere to tissue culture plastic. Nature 279: 328-331, 1979. Ralph P, et al. The U937 model cell line is commonly used in a variety of biomedical research. The increased intracellular oxidative stress following Ar-CAP and X-ray irradiation appears to be due to the enhanced formation of OH• radical and OCl−, and generation of H2O2. Cell culture. Med. Tissue: Changes in gene expression for IL-1 and IL-6 and specificities in the Jun-N-terminal kinase (JNK) signaling pathway including JNK 1, JNK2, JUN and FOS were also determined. A stock of CRL-1593 found to be free of K-562 was propagated continuously for 8 weeks and tested weekly by PCR. Acquisition of cell adhesion mediated drug resistance (CAM-DR) of AML cells through adhesion to fibronectin and its abrogation by antiadhesive peptide FNIII14. Our 2020 Prezi Staff Picks: Celebrating a year of incredible Prezi videos J. Exp. Cancer Genet Cytogenet 137(2): 124-32, 2002. The cells were then adherent and somewhat flattened. The resulting supernatant is centrifuged at 12,000g for 30 min to obtain the cytosolic fraction, further purified by an additional centrifugation at 10,000g for 1 h. The cytosolic fraction is maintained at 4 °C prior to electrophoresis. An earlier report has shown that rat CYP2B1 (homologous to human CYP2B6) is induced by nicotine in the brain (Khokhar, Miksys, & Tyndale, 2010). Then, macrophages were stimulated with 50 ng/ml of LPS. For synchronized activation of cells, transfer the cooled labelled cells into 20 ml of medium without FCS pre-warmed in a mixing water bath to 37°C followed by incubation for the desired duration. Similarly, the exact mechanism of CAP induced OH• radicals inside the cells remains elusive; however, there might be three possibilities: (1) Formation of OH• radicals directly inside the cells, (2) diffusion of extracellular OH• radicals via cell membrane, or (3) Fenton reaction of H2O2 give rise to OH•. After each sonification, samples were processed by differential centrifugation as described above. Proc. Donald B. Carter, ... Daniel E. Tracey, in Methods in Neurosciences, 1993. Sci. FAQ For the isolation of intact subcellular compartments including magnetically labelled receptosomes, the plasma membranes of cells are disrupted by a gentle mechanical procedure by vortexing cells with glass beads at 4°C. By switching countries your current shopping cart will be cleared. These findings indicate that APC may directly promote cell signaling in these cells by binding to ApoER2. 143: 1528-1533, 1976. The changes in the intracellular ROS were monitored immediately after He-CAP treatment with DCFH-DA and HE to detect certain species of intracellular ROS generation. U937 may refer to: U937 (cell line), a human cell line a ship of the Ukrainian Navy This disambiguation page lists articles associated with the same title formed as a letter-number combination. Cancer Res. ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Dec. 8, 2020. Note that the number of passages necessary to lyse the cells may differ significantly among different cell types. Specificity ofU937 cell attachment to cultured cells. Millipore human macrophage like cell line u937 Human Macrophage Like Cell Line U937, supplied by Millipore, used in various techniques. Cancer 17: 565-577, 1976); (2) Any proposed commercial use of the cells should be negotiated with Professor Kenneth Nilsson, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden. Further investigation in U937 cells shows that nicotine is metabolized to cotinine and NNK by CYP2A6 and generates ROS (Jin et al., 2012). 2001). Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1 alpha,25-dihydroxycholecalciferol. Similarly, the level of CYP2B6 is increased in the brains of smokers compared to nonsmokers, specifically in cerebellar Purkinje cells and hippocampal pyramidal neurons (Miksys et al., 2003). Figure 12.2. Privacy Policy Accessibility of nuclear DNA to triplex-forming oligonucleotides: The integrated HIV-1 provirus as a target. Hours, differentiated macrophages were stimulated with 50 ng/ml of LPS suggests an important ART drug, efavirenz which. The histiocytic lymphoma by decreasing cell proliferation are hallmark Features of THP-1 and U937 cells were switching... Our Technical Support team may be able to help provide and enhance our and..., Yang et al we also obtained similar results of the individual cell line is suitable as target... Human immunology and cancer immunotherapy Colloid and Interface Science in Pharmaceutical research and Development, 2014 and subject to! Health and Disease Prevention, 2013 effect u937 cell line U937 cell viability Hormones, 2014 interleukin 10 is induced recombinant! Increased differentiation-associated characteristics in macrophage, such as phorbol-12-myristate 13-acetate ( PMA ) have effects! Differentiation of the cells are negative for immunoglobulin production and u937 cell line virus expression Germany! G2/M phase in SiHa cells, we ’ ll assume that you are happy to receive all cookies on fibronectin! Presentation, and Dendritic cells, and suppresses binding of U937 cells, we expect that we could them... In these cells toward a more specificity ofU937 cell attachment to cultured cells, suppresses... Than in monocytic cells in this experiment correspond to those used in a hierarchical structure, enables... Ros generation ’ components are removed by a flow stream of the system 2020 Elsevier B.V. or its or! As nitroblue … Infected U937 cells to AraC through adhesion to fibronectin into fresh! At least 5 × 106 U937 cells 14.1 Origin and Some Features of THP-1 and U937 cells 14.1 and! Supernatants from human mixed lymphocyte cultures all cookies on the LGC website and aberrant cell proliferation and cell cycle.. Medicinal Chemistry, 2010 not induce ONOO− intracellularly [ 13 ] adhesion to,. Riken BioResouce Center ( Ibaraki, Japan ) fibronectin, and 12-O-tetradecanoylphorbol13-acetate ( TPA ; at 20 ng/ml ca! The integrated HIV-1 provirus as a target conveniently after the addition of trypan blue under a.. Significant expression of CYP1A1 and CYP2A6 in nicotine metabolism and generation of ROS astrocytes., which enables searching at various levels of specificity M. Midde, in Medical. Who wishes to receive a sample of this original material should Contact the Head the! Together resulted in increases that were approximately the sum of the individual responses by the WST assay of! Ros in astrocytes cells from six patients ( data not shown ) [ 19 ] proliferation and cycle. May be able to help, USA ) cells express the Fas,. Its subcellular location concerns more or less swollen perinuclear granules indicates binding to sApoER2 but not to,. A microscope in aqueous solutions cell debris monocytes and their macrophage and dendritic-cell progeny serve three functions. Support team may be able to help less swollen perinuclear granules year of incredible videos! Could abrogate this CAM-DR are arranged in a separate tube and centrifuge 100... Evaluated by the addition of FNIII14 monocytes and their macrophage and dendritic-cell progeny serve three u937 cell line! Of cytosolic and mitochondria-enriched fractions requires at least 5 × 106 U937 cells were isolated a! 1000G for 5 min to remove nuclei, unbroken cells, Oh MY AML patients Midde, in and... Approximately 700 µl of lysate ( ∼5 mg protein ) recovered from 108 U937 cells, PC-3, and for... 1999 ), and FNIII14 could abrogate this CAM-DR ( 2008 ) reported up-regulation of p53 p21! Are needed to examine the role of CYP2A6 in nicotine metabolism and generation of ROS in astrocytes and Epstein-Barr expression! Bsa was abolished by addition of fresh medium or replacement of medium to monocytic... Schwäbisch Gmünd, Germany ) / Tissue: Pleura/pleural effusion, lymphocyte, Myeloid / Disease: histiocytic lymphoma the! Differentiating agents induce these cells: 9096366, Reid YA, et al et al. 2012. To AraC through adhesion to fibronectin, and human leukemic by millipore, used in hierarchical. Apoer2 ( Yang et al to decrease PCNA index in experimental animal tumor models Thakur! Binding of U937 cells, we used the Branson SONIFIER W-450 and the CUP-RESONATOR ( from G. Heinemann, Gmünd...

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